Isolation of actinomycetes from peatland to suppress the growth of Ganoderma boninense the causal agent of basal stem rot disease in oil palm

Abstract. Budi MBS, Giyanto, Tondok ET. 2022. Isolation of actinomycetes from peatland to suppress the growth Ganoderma boninense causal agent basal stem rot disease in oil palm. Biodiversitas 23: 5914-5922. Basal is a major constrain palm cultivation. The aim this study was obtain isolates that can boninense. research stages included rejuvenation isolate stock cultures, observation morphological characters, analysis potency as biological control agents against G. (antibiosis, volatile organic compound, effect bioactive compounds actinomycete culture supernatants and their concentration levels suppressing boninense), physiological characterization molecular identification also done selected actinomycetes. All colonies were grey brown color with rectiflexible vertilicillate spore chain types. Three seven tested, namely AKT19, AKT28, AKT52, able up 100% based on dual test percent inhibition 12.27; 17.50; 23.23% by compounds. At 10% concentration, three inhibited boninense, i.e., 89.17, 88.30 86.50%, respectively. Further results revealed AKT19 strongest suppressor (70.93) at 0.5%, while AKT 28 52 only 15.30 4.80%, potential produce chitinase, glucanase, cellulase, phosphatase enzymes, fixes nitrogen secreted indole acetic acid hormone. Based 16S rRNA gene, it noted all identified Streptomyces gelaticus.